Combined GM-CSF and G-CSF administration mobilizes CD4⁺ CD25^hi Foxp3^hi Treg in leukapheresis products of rhesus monkeys.

ABSTRACT: Early phase clinical trials are evaluating the feasibility, safety, and therapeutic potential of ex vivo expanded regulatory T cells (Treg) in transplantation. A limitation is the paucity of naturally occurring Treg numbers in peripheral blood. Hence, protracted ex vivo expansion is required to obtain sufficient Treg in order to meet target cell doses. Because cytokine administration has been used successfully to mobilize immune cells to the peripheral blood in experimental and clinical studies, we hypothesized that granulocyte macrophage-colony-stimulating factor (GM-CSF) and granulocyte-CSF (G-CSF) administration would enhance Treg percentages in leukapheresis products of rhesus monkeys. Following combined GM-CSF and G-CSF administration, the incidence of Treg in peripheral blood and leu

SUBMITTER: Sasaki K 

PROVIDER: S-EPMC7768825 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature