Bacteriophage-Mediated Reduction of Bacterial Speck on Tomato Seedlings.

ABSTRACT: Background: One crucial first step in bacteriophage therapy is choosing a phage to apply, which involves screening for effectiveness in a meaningful way. Increasingly, research suggests that in vitro tests of phage-mediated bacterial lysis poorly translate to in planta effectiveness. Materials and Methods: We tested a seedling-based method for rapidly screening phage effectiveness in vivo. In three trials, phages were prophylactically applied to tomato seedlings in sterile conical tubes before flooding with the bacterial pathogen Pseudomonas syringae pv. tomato DC3000. We recorded seedling disease progression and quantified endpoint bacteria and phage densities. Results: Phages replicated in all trials, but reduct

SUBMITTER: Hernandez CA 

PROVIDER: S-EPMC7768991 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature