Ectodomain shedding by ADAM17 (a disintegrin and metalloproteinase 17) in canine neutrophils.

ABSTRACT: ADAM17 is a transmembrane protease expressed by most cells in humans and mice that cleaves cell surface substrates primarily in a cis manner, a process referred to as ectodomain shedding. ADAM17 has numerous substrates and plays a broad role in various physiological processes, including as a key regulator of inflammation. At this time, little is known about ADAM17 expression and function in dogs. A well-established ADAM17 substrate is the leukocyte adhesion protein CD62L (L-selectin). We show that a selective inhibitor of ADAM17, but not an inhibitor of its most closely related family member ADAM10, blocks CD62L shedding upon canine neutrophil activation. We also tested several anti-human ADAM17 monoclonal antibodies (mAbs) for staining canine neutrophils. Although most did not recognize c

SUBMITTER: Snyder KM 

PROVIDER: S-EPMC7769933 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature