Single-cell RNA sequencing reveals regulation of fetal ovary development in the monkey (Macaca fascicularis).

ABSTRACT: Germ cells are vital for reproduction and heredity. However, the mechanisms underlying female germ cell development in primates, especially in late embryonic stages, remain elusive. Here, we performed single-cell RNA sequencing of 12,471 cells from whole fetal ovaries, and explored the communications between germ cells and niche cells. We depicted the two waves of oogenesis at single-cell resolution and demonstrated that progenitor theca cells exhibit similar characteristics to Leydig cells in fetal monkey ovaries. Notably, we found that ZGLP1 displays differentially expressed patterns between mouse and monkey, which is not overlapped with NANOG in monkey germ cells, suggesting its role in meiosis entry but not in activating oogenic program in primates. Furthermore, the majority of germ ce

SUBMITTER: Zhao ZH 

PROVIDER: S-EPMC7769980 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature