Human apolipoprotein A-II reduces atherosclerosis in knock-in rabbits.

ABSTRACT:

Background and aims

Apolipoprotein A-II (apoAII) is the second major apolipoprotein of the high-density lipoprotein (HDL) particle, after apoAI. Unlike apoAI, the biological and physiological functions of apoAII are unclear. We aimed to gain insight into the specific roles of apoAII in lipoprotein metabolism and atherosclerosis using a novel rabbit model.

Methods

Wild-type (WT) rabbits are naturally deficient in apoAII, thus their HDL contains only apoAI. Using TALEN technology, we replaced the endogenous apoAI in rabbits through knock-in (KI) of human apoAII. The newly generated apoAII KI rabbits were used to study the specific function of apoAII, independent of apoAI.

Results

ApoAII KI rabbits expressed exclusively apoAII without apoAI, as confirmed by RT-PCR and We

SUBMITTER: Koike T 

PROVIDER: S-EPMC7770079 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature