Microglial Calcium Waves During the Hyperacute Phase of Ischemic Stroke.

ABSTRACT:

Background and purpose

Ischemic injury triggers multiple pathological responses in the brain tissue, including spreading depolarizations across the cerebral cortex (cortical spreading depolarizations [CSD]). Microglia have been recently shown to play a significant role in the propagation of CSD. However, the intracellular responses of myeloid cells during ischemic stroke have not been investigated.

Methods

We have studied intracellular calcium activity in cortical microglia in the stroke model of the middle cerebral artery occlusion, using the murine Polr2a-based and Cre-dependent GCaMP5 and tdTomato reporter (PC::G5-tdT). High-speed 2-photon microscopy through cranial windows was employed to record signals from genetically encoded indicators of calcium. Inflammatory stimuli

SUBMITTER: Liu L 

PROVIDER: S-EPMC7770090 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature