Glycosylation of Ca_V3.2 Channels Contributes to the Hyperalgesia in Peripheral Neuropathy of Type 1 Diabetes.

ABSTRACT: Our previous studies implicated glycosylation of the Ca_V3.2 isoform of T-type Ca²⁺ channels (T-channels) in the development of Type 2 painful peripheral diabetic neuropathy (PDN). Here we investigated biophysical mechanisms underlying the modulation of recombinant Ca_V3.2 channel by de-glycosylation enzymes such as neuraminidase (NEU) and PNGase-F (PNG), as well as their behavioral and biochemical effects in painful PDN Type 1. In our in vitro study we used whole-cell recordings of current-voltage relationships to confirm that Ca_V3.2 current densities were decreased ~2-fold after de-glycosylation. Furthermore, de-glycosylation induced a significant depolarizing shift in the steady-state relationships for activation and inactivation while pr

SUBMITTER: Joksimovic SL 

PROVIDER: S-EPMC7770106 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature