Mitochondrial Ca²⁺ Overload Leads to Mitochondrial Oxidative Stress and Delayed Meiotic Resumption in Mouse Oocytes.

ABSTRACT: Overweight or obese women seeking pregnancy is becoming increasingly common. Human maternal obesity gives rise to detrimental effects during reproduction. Emerging evidence has shown that these abnormities are likely attributed to oocyte quality. Oxidative stress induces poor oocyte conditions, but whether mitochondrial calcium homeostasis plays a key role in oocyte status remains unresolved. Here, we established a mitochondrial Ca²⁺ overload model in mouse oocytes. Knockdown gatekeepers of the mitochondrial Ca²⁺ uniporters Micu1 and Micu2 as well as the mitochondrial sodium calcium exchanger NCLX in oocytes both increased oocytes mitochondrial Ca²⁺ concentration. The overload of mitochondria Ca²⁺ in oocytes impaired mitochondrial function, leade

SUBMITTER: Zhang L 

PROVIDER: S-EPMC7770107 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature