Verteporfin treatment controls morphology, phenotype, and global gene expression for cells of the human nucleus pulposus.

ABSTRACT: Cells of the nucleus pulposus (NP) are essential contributors to extracellular matrix synthesis and function of the intervertebral disc. With age and degeneration, the NP becomes stiffer and more dehydrated, which is associated with a loss of phenotype and biosynthetic function for its resident NP cells. Also, with aging, the NP cell undergoes substantial morphological changes from a rounded shape with pronounced vacuoles in the neonate and juvenile, to one that is more flattened and spread with a loss of vacuoles. Here, we make use of the clinically relevant pharmacological treatment verteporfin (VP), previously identified as a disruptor of yes-associated protein-TEA domain family member-binding domain (TEAD) signaling, to promote morphological changes in adult human NP cells in order to

SUBMITTER: Fearing BV 

PROVIDER: S-EPMC7770208 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature