Rab27b contributes to radioresistance and exerts a paracrine effect via epiregulin in glioblastoma.

ABSTRACT:

Background

Radiotherapy is the standard treatment for glioblastoma (GBM). However, radioresistance of GBM cells leads to recurrence and poor patient prognosis. Recent studies suggest that secretion factors have important roles in radioresistance of tumor cells. This study aims to determine whether Rab27b, a small GTPase involved in secretory vesicle trafficking, plays a role in radioresistance of GBM.

Methods

Microarray analysis, cell viability analysis, apoptosis assay, immunostaining, and in vivo experiments were performed to assess the effect of Rab27b on radioresistance of GBM. We further investigated paracrine effects mediated by Rab27b after X-ray irradiation using coculture systems of glioma cell lines.

Results

Rab27b was specifically upregulated in irradiated

SUBMITTER: Nishioka S 

PROVIDER: S-EPMC7770522 | biostudies-literature | 2020 Jan-Dec

REPOSITORIES: biostudies-literature