Dynamics of DNA Clogging in Hafnium Oxide Nanopores.

ABSTRACT: Interfacing solid-state nanopores with biological systems has been exploited as a versatile analytical platform for analysis of individual biomolecules. Although clogging of solid-state nanopores due to nonspecific interactions between analytes and pore walls poses a persistent challenge in attaining the anticipated sensing efficacy, insufficient studies focus on elucidating the clogging dynamics. Herein, we investigate the DNA clogging behavior by passing double-stranded (ds) DNA molecules of different lengths through hafnium oxide(HfO₂)-coated silicon (Si) nanopore arrays, at different bias voltages and electrolyte pH values. Employing stable and photoluminescent-free HfO₂/Si nanopore arrays permits a parallelized visualization of DNA clogging with confocal fluoresc

SUBMITTER: Li S 

PROVIDER: S-EPMC7770817 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature