Differential alternative splicing between hepatocellular carcinoma with normal and elevated serum alpha-fetoprotein.

ABSTRACT:

Background

Serum alpha-fetoprotein (AFP) is the approved serum marker for hepatocellular carcinoma (HCC) screening. However, not all HCC patients show high (≥ 20 ng/mL) serum AFP, and the molecular mechanisms of HCCs with normal (< 20 ng/mL) serum AFP remain to be elucidated. Therefore, we aimed to identify biological features of HCCs with normal serum AFP by investigating differential alternative splicing (AS) between HCCs with normal and high serum AFP.

Methods

We performed a genome-wide survey of AS events in 249 HCCs with normal (n = 131) and high (n = 118) serum AFP levels using RNA-sequencing data obtained from The Cancer Genome Atlas.

Results

In group comparisons of RNA-seq profiles from HCCs with normal and high serum AFP levels, 161 differential AS events (12

SUBMITTER: Jin YJ 

PROVIDER: S-EPMC7771076 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature