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Sequential Quadriplex Real-Time PCR for Identifying 20 Common emm Types of Group A Streptococcus.

ABSTRACT: We developed a sequential quadriplex real-time PCR-based method for rapid identification of 20 emm types commonly found in invasive group A Streptococcus (iGAS) strains recovered through the Centers for Disease Control and Prevention's Active Bacterial Core surveillance. Each emm real-time PCR assay showed high specificity and accurately identified the respective target emm type, including emm subtypes in the United States. Furthermore, this method is useful for rapid typing of GAS isolates and culture-negative specimens during outbreak investigations.

SUBMITTER: Velusamy S

PROVIDER: S-EPMC7771451 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature

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Sequential Quadriplex Real-Time PCR for Identifying 20 Common emm Types of Group A Streptococcus.

Velusamy Srinivasan S Jordak Katherine K Kupor Madeline M Chochua Sopio S McGee Lesley L Beall Bernard B

Journal of clinical microbiology 20201217 1

We developed a sequential quadriplex real-time PCR-based method for rapid identification of 20 emm types commonly found in invasive group A Streptococcus (iGAS) strains recovered through the Centers for Disease Control and Prevention's Active Bacterial Core surveillance. Each emm real-time PCR assay showed high specificity and accurately identified the respective target emm type, including emm subtypes in the United States. Furthermore, this method is useful fo ...[more]

PMID: 33087429

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