Spatial Multiplexing of Fluorescent Reporters for Imaging Signaling Network Dynamics.

ABSTRACT: In order to analyze how a signal transduction network converts cellular inputs into cellular outputs, ideally one would measure the dynamics of many signals within the network simultaneously. We found that, by fusing a fluorescent reporter to a pair of self-assembling peptides, it could be stably clustered within cells at random points, distant enough to be resolved by a microscope but close enough to spatially sample the relevant biology. Because such clusters, which we call signaling reporter islands (SiRIs), can be modularly designed, they permit a set of fluorescent reporters to be efficiently adapted for simultaneous measurement of multiple nodes of a signal transduction network within single cells. We created SiRIs for indicators of second messengers and kinases and used them, in hip

SUBMITTER: Linghu C 

PROVIDER: S-EPMC7771521 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature