JUUL e-liquid exposure elicits cytoplasmic Ca²⁺ responses and leads to cytotoxicity in cultured airway epithelial cells.

ABSTRACT:

Rationale

The popularity of new and emerging tobacco products such as E-cigarettes (E-cigs) is rapidly expanding worldwide. However, uncertainties surrounding the potential health consequences due to the use of such products exist and warrant further study.

Methods

Cultured A549 and Calu-3 airway epithelia were exposed to three out of the eight types of JUUL brand e-liquids ("Mint", "Virginia Tobacco" and "Menthol", all containing 3% nicotine at 1% and 3% (vol/vol) dilutions) and assessed for viability using a resazurin-based assay. Intracellular Ca²⁺ levels were measured using fluorescent indicators and pro-inflammatory cytokine levels were monitored by quantitative PCR (qPCR). Cultures were also analyzed by flow cytometry to evaluate apoptotic markers and cell v

SUBMITTER: Zhang R 

PROVIDER: S-EPMC7772262 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature