Characterization of putative interneurons in the myenteric plexus of human colon.

ABSTRACT:

Background

The enteric nervous system contains multiple classes of neurons, distinguishable by morphology, immunohistochemical markers, and projections; however, specific combinations differ between species. Here, types of enteric neurons in human colon were characterized immunohistochemically, using retrograde tracing combined with multiple labeling immunohistochemistry, focussing on non-motor neurons.

Methods

The fluorescent carbocyanine tracer, DiI, was applied to the myenteric plexus in ex vivo preparations, filling neurons projecting within the plexus. Limits of projection lengths of motor neurons were established, allowing them to be excluded from the analysis. Long ascending and descending interneurons were then distinguished by labeling for discriminating immunohisto

SUBMITTER: Humenick A 

PROVIDER: S-EPMC7772282 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature