Functional Prediction and Assignment of Methanobrevibacter ruminantium M1 Operome Using a Combined Bioinformatics Approach.

ABSTRACT: Methanobrevibacter ruminantium M1 (MRU) is a rod-shaped rumen methanogen with the ability to use H₂ and CO₂, and formate as substrates for methane formation in the ruminants. Enteric methane emitted from this organism can also be influential to the loss of dietary energy in ruminants and humans. To date, there is no successful technology to reduce methane due to a lack of knowledge on its molecular machinery and 73% conserved hypothetical proteins (HPs; operome) whose functions are still not ascertained perceptively. To address this issue, we have predicted and assigned a precise function to HPs and categorize them as metabolic enzymes, binding proteins, and transport proteins using a combined bioinformatics approach. The results of our study show that 257 (34%

SUBMITTER: Bharathi M 

PROVIDER: S-EPMC7772410 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature