Mapping germ-layer specification preventing genes in hPSCs via genome-scale CRISPR screening.

ABSTRACT: Understanding the biological processes that determine the entry of three germ layers of human pluripotent stem cells (hPSCs) is a central question in developmental and stem cell biology. Here, we genetically engineered hPSCs with the germ layer reporter and inducible CRISPR/Cas9 knockout system, and a genome-scale screening was performed to define pathways restricting germ layer specification. Genes clustered in the key biological processes, including embryonic development, mRNA processing, metabolism, and epigenetic regulation, were centered in the governance of pluripotency and lineage development. Other than typical pluripotent transcription factors and signaling molecules, loss of function of mesendodermal specifiers resulted in advanced neuroectodermal differentiation, given their int

SUBMITTER: Xu X 

PROVIDER: S-EPMC7772566 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature