Promoter P _PSP1-5-BnPSP-1 From Ramie (Boehmeria nivea L. Gaud.) Can Drive Phloem-Specific GUS Expression in Arabidopsis thaliana.

ABSTRACT: Isolation of phloem-specific promoters is one of the basic conditions for improving the fiber development and resistance of ramie phloem using genetic engineering. In this study, we isolated a ramie endogenous promoter (named P _PSP1 -BnPSP-1) and analyzed the function of its truncated fragments in Arabidopsis. The results show that P _PSP1 -BnPSP-1 can drive the GUS reporter gene to be specifically expressed in the veins of Arabidopsis. After hormone and simulated drought treatment of the independent Arabidopsis lines carrying P _PSP1 -BnPSP-1 and its truncated fragments, only P _PSP1-5-BnPSP-1 (-600 to -1 bp region of P _PSP1 -BnPSP-1) is stably expressed and exhibits p

SUBMITTER: Wang Y 

PROVIDER: S-EPMC7772962 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature