Inhibition of the replication of SARS-CoV-2 in human cells by the FDA-approved drug chlorpromazine.

ABSTRACT:

Introduction

Urgent action is needed to fight the ongoing coronavirus disease 2019 (COVID-19) pandemic by reducing the number of infected cases, contagiousness and severity. Chlorpromazine (CPZ), an antipsychotic from the phenothiazine group, is known to inhibit clathrin-mediated endocytosis and has antiviral activity against severe acute respiratory syndrome coronavirus-1 (SARS-CoV-1) and Middle East respiratory syndrome coronavirus. The aim of this in-vitro study was to test CPZ against SARS-CoV-2 in monkey and human cells.

Materials and methods

Monkey VeroE6 cells and human alveolar basal epithelial A549-ACE2 cells were infected with SARS-CoV-2 in the presence of various concentrations of CPZ. Supernatants were harvested at day 2 and analysed by quantitative reverse trans

SUBMITTER: Plaze M 

PROVIDER: S-EPMC7772996 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature