FLIMJ: An open-source ImageJ toolkit for fluorescence lifetime image data analysis.

ABSTRACT: In the field of fluorescence microscopy, there is continued demand for dynamic technologies that can exploit the complete information from every pixel of an image. One imaging technique with proven ability for yielding additional information from fluorescence imaging is Fluorescence Lifetime Imaging Microscopy (FLIM). FLIM allows for the measurement of how long a fluorophore stays in an excited energy state, and this measurement is affected by changes in its chemical microenvironment, such as proximity to other fluorophores, pH, and hydrophobic regions. This ability to provide information about the microenvironment has made FLIM a powerful tool for cellular imaging studies ranging from metabolic measurement to measuring distances between proteins. The increased use of FLIM has necessitated

SUBMITTER: Gao D 

PROVIDER: S-EPMC7773231 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature