Functionally specialized human CD4+ T-cell subsets express physicochemically distinct TCRs.

ABSTRACT: The organizational integrity of the adaptive immune system is determined by functionally discrete subsets of CD4+ T cells, but it has remained unclear to what extent lineage choice is influenced by clonotypically expressed T-cell receptors (TCRs). To address this issue, we used a high-throughput approach to profile the αβ TCR repertoires of human naive and effector/memory CD4+ T-cell subsets, irrespective of antigen specificity. Highly conserved physicochemical and recombinatorial features were encoded on a subset-specific basis in the effector/memory compartment. Clonal tracking further identified forbidden and permitted transition pathways, mapping effector/memory subsets related by interconversion or ontogeny. Public sequences were largely confined to particular effector/memory subsets,

SUBMITTER: Kasatskaya SA 

PROVIDER: S-EPMC7773335 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature