Hybrid methods for combined experimental and computational determination of protein structure.

ABSTRACT: Knowledge of protein structure is paramount to the understanding of biological function, developing new therapeutics, and making detailed mechanistic hypotheses. Therefore, methods to accurately elucidate three-dimensional structures of proteins are in high demand. While there are a few experimental techniques that can routinely provide high-resolution structures, such as x-ray crystallography, nuclear magnetic resonance (NMR), and cryo-EM, which have been developed to determine the structures of proteins, these techniques each have shortcomings and thus cannot be used in all cases. However, additionally, a large number of experimental techniques that provide some structural information, but not enough to assign atomic positions with high certainty have been developed. These methods offer

SUBMITTER: Seffernick JT 

PROVIDER: S-EPMC7773420 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature