Structural Differences in Translation Initiation between Pathogenic Trypanosomatids and Their Mammalian Hosts.

ABSTRACT: Canonical mRNA translation in eukaryotes begins with the formation of the 43S pre-initiation complex (PIC). Its assembly requires binding of initiator Met-tRNA_i^Met and several eukaryotic initiation factors (eIFs) to the small ribosomal subunit (40S). Compared to their mammalian hosts, trypanosomatids present significant structural differences in their 40S, suggesting substantial variability in translation initiation. Here, we determine the structure of the 43S PIC from Trypanosoma cruzi, the parasite causing Chagas disease. Our structure shows numerous specific features, such as the variant eIF3 structure and its unique interactions with the large rRNA expansion segments (ESs) 9^S, 7^S, and 6^S, and the association of a kinetoplastid-spec

SUBMITTER: Bochler A 

PROVIDER: S-EPMC7773551 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature