Three-Dimensional Analysis of Busulfan-Induced Spermatogenesis Disorder in Mice.

ABSTRACT: We examined if the distribution of impaired or normal spermatogenesis differs along the length of seminiferous tubules in disorders of spermatogenesis. For this purpose, three-dimensional (3D) reconstruction of seminiferous tubules was performed in mice with experimental spermatogenesis disorder induced by intraperitoneal injection of busulfan, and the areas of impaired and normal spermatogenesis were analyzed microscopically. The volume of the testis and length of seminiferous tubules decreased, and the proportion of tubule areas with impaired spermatogenesis increased depending on the dose of busulfan. With the highest dose of busulfan, although the proportion of impaired spermatogenesis was similar among individual seminiferous tubules, it was slightly but significantly higher in shorte

SUBMITTER: Nakata H 

PROVIDER: S-EPMC7773783 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature