Diverting tyrosine: Data from untargeted metabolic analysis of tomato fruit accumulating L-DOPA.

ABSTRACT: L-DOPA, also known as Levodopa or L-3,4-dihydroxyphenylalanine, is synthesised in plants from the amino acid tyrosine, through oxidation. Conversion of tyrosine to L-DOPA constitues the first step of betalain biosynthesis in plants. Recently, the gene responsible for this step was identified in beetroot, BvCYP76AD6, that is the source of yellow and purple betalain pigments. Overexpression of this gene, specifically in tomato fruit, led to accumulation of L-DOPA that otherwise is not detectable [1]. Co-expression of the Arabidopsis transcription factor, AtMYB12, in fruit, increased L-DOPA levels further. To study the metabolic changes in these fruit, we performed untargeted metabolite analysis of ripe fruit: GC-MS was performed to identify changes in primary metabolites, LC-MS

SUBMITTER: Breitel D 

PROVIDER: S-EPMC7773851 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature