Matching is the Key Factor to Improve the Production of Patchoulol in the Plant Chassis of Marchantia paleacea.

ABSTRACT: The valuable terpenoids, such as artemisinin acid, have achieved bioproduction in the chassis of microbes recently. In this study, Marchantia paleacea L, a promising plant synthetic biology chassis, was used to explore the possibility of patchoulol production by constructing a synthetic biology pathway composed of FPS and PTS. The experiment results show that the maximum yields based on the cytoplasm and plastid pathway were 621.56 and 1006.45 μg/g, respectively. However, there is no statistically significant difference in the yield of patchoulol between transformant plants with different subcellular compartment-targeting pathways. However, it was found that the highest yield of patchoulol was achieved in transformant plants with similar transcription levels of FPS<

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC7774073 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature