Klf14 is an imprinted transcription factor that regulates placental growth.

ABSTRACT:

Introduction

Imprinted genes are preferentially expressed from one parentally inherited allele, and many are crucial to the regulation of placental function and fetal growth. Murine Krüppel-like factor 14 (Klf14) is a maternally expressed imprinted transcription factor that is a component of the Mest imprinted gene cluster on mouse chromosome 6. We sought to determine if loss of Klf14 expression alters the course of normal mouse extraembryonic development. We also used high-throughput RNA sequencing (RNAseq) to identify a set of differentially expressed genes (DEGs) in placentas with loss of Klf14.

Methods

We generated a Klf14 knockout (Klf14^null) mouse using recombineering and transgenic approaches. To identify DEGs in the mouse placenta we compared mRNA transcri

SUBMITTER: Koppes E 

PROVIDER: S-EPMC7774094 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature