Selection of High-Affinity RNA Aptamers That Distinguish between Doxycycline and Tetracycline.

ABSTRACT: Oligonucleotide aptamers are found in prokaryotes and eukaryotes, and they can be selected from large synthetic libraries to bind protein or small-molecule ligands with high affinities and specificities. Aptamers can function as biosensors, as protein recognition elements, and as components of riboswitches allowing ligand-dependent control of gene expression. One of the best studied laboratory-selected aptamers binds the antibiotic tetracycline, but it binds with a much lower affinity to the closely related but more bioavailable antibiotic doxycycline. Here we report enrichment of doxycycline binding aptamers from a selectively randomized library of tetracycline aptamer variants over four selection rounds. Selected aptamers distinguish between doxycycline, which they bind with dissociation

SUBMITTER: Tickner ZJ 

PROVIDER: S-EPMC7774328 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature