SlitC-PlexinA1 mediates iterative inhibition for orderly passage of spinal commissural axons through the floor plate.

ABSTRACT: Spinal commissural axon navigation across the midline in the floor plate requires repulsive forces from local Slit repellents. The long-held view is that Slits push growth cones forward and prevent them from turning back once they became sensitized to these cues after midline crossing. We analyzed with fluorescent reporters Slits distribution and FP glia morphology. We observed clusters of Slit-N and Slit-C fragments decorating a complex architecture of glial basal process ramifications. We found that PC2 proprotein convertase activity contributes to this pattern of ligands. Next, we studied Slit-C acting via PlexinA1 receptor shared with another FP repellent, the Semaphorin3B, through generation of a mouse model baring PlexinA1_Y1815F mutation abrogating SlitC but not Sema3B res

SUBMITTER: Ducuing H 

PROVIDER: S-EPMC7775108 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature