Lysis of cold-storage-induced microvascular obstructions for ex vivo revitalization of marginal human kidneys.

ABSTRACT: Thousands of kidneys from higher-risk donors are discarded annually because of the increased likelihood of complications posttransplant. Given the severe organ shortage, there is a critical need to improve utilization of these organs. To this end, normothermic machine perfusion (NMP) has emerged as a platform for ex vivo assessment and potential repair of marginal organs. In a recent study of 8 transplant-declined human kidneys on NMP, we discovered microvascular obstructions that impaired microvascular blood flow. However, the nature and physiologic impact of these lesions were unknown. Here, in a study of 39 human kidneys, we have identified that prolonged cold storage of human kidneys induces accumulation of fibrinogen within tubular epithelium. Restoration of normoxic conditions-either

SUBMITTER: DiRito JR 

PROVIDER: S-EPMC7775334 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature