Quantitative and simultaneous measurement of oxygen consumption rates in rat brain and skeletal muscle using ¹⁷ O MRS imaging at 16.4T.

ABSTRACT:

Purpose

Oxygen-17 (¹⁷ O) MRS imaging, successfully used in the brain, is extended by imaging the oxygen metabolic rate in the resting skeletal muscle and used to determine the total whole-body oxygen metabolic rate in the rat.

Methods

During and after inhalations of ¹⁷ O₂ gas, dynamic ¹⁷ O MRSI was performed in rats (n = 8) ventilated with N₂ O or N₂ at 16.4 T. Time courses of the H₂¹⁷ O concentration from regions of interest located in brain and muscle tissue were examined and used to fit an animal-adapted 3-phase metabolic model of oxygen consumption. CBF was determined with an independent washout method. Finally, body oxygen metabolic rate was calculated using a global steady-state app

SUBMITTER: Wiesner HM 

PROVIDER: S-EPMC7775343 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature