Specific Induction of Double Negative B Cells During Protective and Pathogenic Immune Responses.

ABSTRACT: Double negative (DN) (CD19⁺CD20^lowCD27^-IgD^-) B cells are expanded in patients with autoimmune and infectious diseases; however their role in the humoral immune response remains unclear. Using systematic flow cytometric analyses of peripheral blood B cell subsets, we observed an inflated DN B cell population in patients with variety of active inflammatory conditions: myasthenia gravis, Guillain-Barré syndrome, neuromyelitis optica spectrum disorder, meningitis/encephalitis, and rheumatic disorders. Furthermore, we were able to induce DN B cells in healthy subjects following vaccination against influenza and tick borne encephalitis virus. Transcriptome analysis revealed a gene expression profile in DN B cells that clustered with naïve B cells, memo

SUBMITTER: Ruschil C 

PROVIDER: S-EPMC7775384 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature