An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells.

ABSTRACT: Electrophilic compounds originating from nature or chemical synthesis have profound effects on immune cells. These compounds are thought to act by cysteine modification to alter the functions of immune-relevant proteins; however, our understanding of electrophile-sensitive cysteines in the human immune proteome remains limited. Here, we present a global map of cysteines in primary human T cells that are susceptible to covalent modification by electrophilic small molecules. More than 3,000 covalently liganded cysteines were found on functionally and structurally diverse proteins, including many that play fundamental roles in immunology. We further show that electrophilic compounds can impair T cell activation by distinct mechanisms involving the direct functional perturbation and/or degrada

SUBMITTER: Vinogradova EV 

PROVIDER: S-EPMC7775622 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature