Optimizing Accuracy and Depth of Protein Quantification in Experiments Using Isobaric Carriers.

ABSTRACT: The isobaric carrier approach, which combines small isobarically labeled samples with a larger isobarically labeled carrier sample, finds diverse applications in ultrasensitive mass spectrometry analysis of very small samples, such as single cells. To enhance the growing use of isobaric carriers, we characterized the trade-offs of using isobaric carriers in controlled experiments with complex human proteomes. The data indicate that isobaric carriers directly enhance peptide sequence identification without simultaneously increasing the number of protein copies sampled from small samples. The results also indicate strategies for optimizing the amount of isobaric carrier and analytical parameters, such as ion accumulation time, for different priorities such as improved quantification or an in

SUBMITTER: Specht H 

PROVIDER: S-EPMC7775882 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature