Isolation, expression, and characterization of raw starch degrading α-amylase from a marine lake Bacillus megaterium NL3.

ABSTRACT: A land-locked marine lake Kakaban with its significant ecological paramaters provides a unique habitat for bacteria with novel biotechnology potential that uses a diverse array of catalytic agents, including α-amylase. Aiming at the isolation of raw starch degrading α-amylase from marine biodiversity, a gene encoding BmaN2 from a sea anemone associated bacterium Bacillus megaterium NL3 was cloned and expressed in Escherichia coli ArcticExpress (DE3). It comprises an open reading frame of 1,563 nucleotides encoding BmaN2 of 520 amino acids and belongs to the glycoside hydrolase family 13 subfamily 36 (GH13_36). This α-amylase has a maximum activity at pH 6.0 and 60 °C with a specific activity of 28.7 U mg^-1. The BmaN2 activity is enhanced strongly by Ca²⁺

SUBMITTER: Shofiyah SS 

PROVIDER: S-EPMC7776835 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature