Centrally circulating α-klotho inversely correlates with human obesity and modulates arcuate cell populations in mice.

ABSTRACT:

Objective

Our laboratory recently identified the centrally circulating α-klotho protein as a novel hypothalamic regulator of food intake and glucose metabolism in mice. The current study aimed to investigate novel molecular effectors of central α-klotho in the arcuate nucleus of the hypothalamus (ARC), while further deciphering its role regulating energy balance in both humans and mice.

Methods

Cerebrospinal fluid (CSF) was collected from 22 adults undergoing lower limb orthopedic surgeries, and correlations between body weight and α-klotho were determined using an α-klotho enzyme-linked immunosorbent assay (ELISA) kit. To investigate the effects of α-klotho on energy expenditure (EE), 2-day intracerebroventricular (ICV) treatment was performed in diet-induced obesity (DIO)

SUBMITTER: Landry T 

PROVIDER: S-EPMC7777546 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature