Temporal focusing multiphoton microscopy with optimized parallel multiline scanning for fast biotissue imaging.

ABSTRACT: Line scanning-based temporal focusing multiphoton microscopy (TFMPM) has superior axial excitation confinement (AEC) compared to conventional widefield TFMPM, but the frame rate is limited due to the limitation of the single line-to-line scanning mechanism. The development of the multiline scanning-based TFMPM requires only eight multiline patterns for full-field uniform multiphoton excitation and it still maintains superior AEC. The optimized parallel multiline scanning TFMPM is developed, and the performance is verified with theoretical simulation. The system provides a sharp AEC equivalent to the line scanning-based TFMPM, but fewer scans are required. A digital micromirror device is integrated in the TFMPM system and generates the multiline pattern for excitation. Based on the result o

SUBMITTER: Chang CY 

PROVIDER: S-EPMC7778456 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature