A tunable anthranilate-inducible gene expression system for Pseudomonas species.

ABSTRACT: Pseudomonads are among the most common bacteria in soils, limnic ecosystems, and human, animal, or plant host environments, including intensively studied species such as Pseudomonas aeruginosa, P. putida, or P. fluorescens. Various gene expression systems are established for some species, but there is still a need for a simple system that is suitable for a wide range of pseudomonads and that can be used for physiological applications, i.e., with a tuning capacity at lower expression levels. Here, we report the establishment of the anthranilate-dependent P_antA promoter for tunable gene expression in pseudomonads. During studies on P. fluorescens, we constructed an anthranilate-inducible AntR/P_antA-based expression system, named pUCP20-ANT, and used GFP as reporter to a

SUBMITTER: Hoffmann L 

PROVIDER: S-EPMC7778614 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature