Sensitive universal detection of blood parasites by selective pathogen-DNA enrichment and deep amplicon sequencing.

ABSTRACT:

Background

Targeted amplicon deep sequencing (TADS) has enabled characterization of diverse bacterial communities, yet the application of TADS to communities of parasites has been relatively slow to advance. The greatest obstacle to this has been the genetic diversity of parasitic agents, which include helminths, protozoa, arthropods, and some acanthocephalans. Meanwhile, universal amplification of conserved loci from all parasites without amplifying host DNA has proven challenging. Pan-eukaryotic PCRs preferentially amplify the more abundant host DNA, obscuring parasite-derived reads following TADS. Flaherty et al. (2018) described a pan-parasitic TADS method involving amplification of eukaryotic 18S rDNA regions possessing restriction sites only in vertebrates. Using this method,

SUBMITTER: Flaherty BR 

PROVIDER: S-EPMC7778815 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature