SCHEEPDOG: Programming Electric Cues to Dynamically Herd Large-Scale Cell Migration.

ABSTRACT: Directed cell migration is critical across biological processes spanning healing to cancer invasion, yet no existing tools allow real-time interactive guidance over such migration. We present a new bioreactor that harnesses electrotaxis-directed cell migration along electric field gradients-by integrating four independent electrodes under computer control to dynamically program electric field patterns, and hence steer cell migration. Using this platform, we programmed and characterized multiple precise, two-dimensional collective migration maneuvers in renal epithelia and primary skin keratinocyte ensembles. First, we demonstrated on-demand, 90-degree collective turning. Next, we developed a universal electrical stimulation scheme capable of programming arbitrary 2D migration maneuvers suc

SUBMITTER: Zajdel TJ 

PROVIDER: S-EPMC7779114 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature