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Phylogenetic and structural diversity of aromatically dense pili from environmental metagenomes.


ABSTRACT: Electroactive type IV pili, or e-pili, are used by some microbial species for extracellular electron transfer. Recent studies suggest that e-pili may be more phylogenetically and structurally diverse than previously assumed. Here, we used updated aromatic density thresholds (≥9.8% aromatic amino acids, ≤22-aa aromatic gaps and aromatic amino acids at residues 1, 24, 27, 50 and/or 51, and 32 and/or 57) to search for putative e-pilin genes in metagenomes from diverse ecosystems with active microbial metal cycling. Environmental putative e-pilins were diverse in length and phylogeny, and included truncated e-pilins in Geobacter spp., as well as longer putative e-pilins in Fe(II)-oxidizing Betaproteobacteria and Zetaproteobacteria.

SUBMITTER: Bray MS 

PROVIDER: S-EPMC7779115 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature

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Phylogenetic and structural diversity of aromatically dense pili from environmental metagenomes.

Bray Marcus S MS   Wu Jieying J   Padilla Cory C CC   Stewart Frank J FJ   Fowle David A DA   Henny Cynthia C   Simister Rachel L RL   Thompson Katharine J KJ   Crowe Sean A SA   Glass Jennifer B JB  

Environmental microbiology reports 20191121 1


Electroactive type IV pili, or e-pili, are used by some microbial species for extracellular electron transfer. Recent studies suggest that e-pili may be more phylogenetically and structurally diverse than previously assumed. Here, we used updated aromatic density thresholds (≥9.8% aromatic amino acids, ≤22-aa aromatic gaps and aromatic amino acids at residues 1, 24, 27, 50 and/or 51, and 32 and/or 57) to search for putative e-pilin genes in metagenomes from diverse ecosystems with active microbi  ...[more]

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