Simultaneous quantification of protein-DNA interactions and transcriptomes in single cells with scDam&T-seq.

ABSTRACT: Protein-DNA interactions are essential for establishing cell type-specific chromatin architecture and gene expression. We recently developed scDam&T-seq, a multi-omics method that can simultaneously quantify protein-DNA interactions and the transcriptome in single cells. The method effectively combines two existing methods: DNA adenine methyltransferase identification (DamID) and CEL-Seq2. DamID works through the tethering of a protein of interest (POI) to the Escherichia coli DNA adenine methyltransferase (Dam). Upon expression of this fusion protein, DNA in proximity to the POI is methylated by Dam and can be selectively digested and amplified. CEL-Seq2, in contrast, makes use of poly-dT primers to reverse transcribe mRNA, followed by linear amplification through in vitro transcription.

SUBMITTER: Markodimitraki CM 

PROVIDER: S-EPMC7779467 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature