Proteomic and Genomic Methylation Signatures of Idiopathic Subglottic Stenosis.

ABSTRACT:

Objective

Idiopathic subglottic stenosis (iSGS) is a chronic inflammatory condition that causes dyspnea and affects middle-aged women of White race and non-Latino or Hispanic ethnicity. To better characterize its phenotype and pathogenesis, we assessed the proteomic and genomic methylation signatures of subglottic tissue collected from iSGS patients compared to controls.

Study design

Molecular analysis of clinical biospecimens.

Methods

We collected subglottic tissue biopsies from 12 patients during direct laryngoscopy, immediately prior to surgical treatment of iSGS; as well as from 4 age-, sex-, and race/ethnicity-matched control patients undergoing other direct laryngoscopic procedures. We isolated protein and genomic DNA, acquired proteomic data using label-free qu

SUBMITTER: Schoeff SS 

PROVIDER: S-EPMC7779687 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature