Exploring rotavirus proteome to identify potential B- and T-cell epitope using computational immunoinformatics.

ABSTRACT: Rotavirus is the most common cause of acute gastroenteritis in infants and children worldwide. The functional correlation of B- and T-cells to long-lasting immunity against rotavirus infection in the literature is limited. In this work, a series of computational immuno-informatics approaches were applied and identified 28 linear B-cells, 26 conformational B-cell, 44 T_C cell and 40 T_H cell binding epitopes for structural and non-structural proteins of rotavirus. Further selection of putative B and T cell epitopes in the multi-epitope vaccine construct was carried out based on immunogenicity, conservancy, allergenicity and the helical content of predicted epitopes. An in-silico vaccine constructs was developed using an N-terminal adjuvant (RGD motif) followed by

SUBMITTER: Devi YD 

PROVIDER: S-EPMC7779714 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature