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Protocol for generation of lung adenocarcinoma organoids from clinical samples.

ABSTRACT: Human cancer tissue-derived organoids maintain the mutational spectrum and histological characteristics of their parental tumors and thus provide a platform for predicting patients' responses to anticancer drugs. Here, we provide a fully detailed, step-by-step protocol to derive lung adenocarcinoma organoids from primary tumor tissues. Organoid lines can be generated with a success rate of 80% using our protocol. For complete details on the use and execution of this protocol, please refer to Li et al. (2020).

SUBMITTER: Li Z

PROVIDER: S-EPMC7779824 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature

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Protocol for generation of lung adenocarcinoma organoids from clinical samples.

Li Zhichao Z Yu Lei L Chen Dong D Meng Zhaoyue Z Chen Wei W Huang Weiren W

STAR protocols 20201228 1

Human cancer tissue-derived organoids maintain the mutational spectrum and histological characteristics of their parental tumors and thus provide a platform for predicting patients' responses to anticancer drugs. Here, we provide a fully detailed, step-by-step protocol to derive lung adenocarcinoma organoids from primary tumor tissues. Organoid lines can be generated with a success rate of 80% using our protocol. For complete details on the use and execution of this protocol, please refer to Li ...[more]

PMID: 33426535

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