White adipose remodeling during browning in mice involves YBX1 to drive thermogenic commitment.

ABSTRACT:

Objective

Increasing adaptive thermogenesis by stimulating browning in white adipose tissue is a promising method of improving metabolic health. However, the molecular mechanisms underlying this transition remain elusive. Our study examined the molecular determinants driving the differentiation of precursor cells into thermogenic adipocytes.

Methods

In this study, we conducted temporal high-resolution proteomic analysis of subcutaneous white adipose tissue (scWAT) after cold exposure in mice. This was followed by loss- and gain-of-function experiments using siRNA-mediated knockdown and CRISPRa-mediated induction of gene expression, respectively, to evaluate the function of the transcriptional regulator Y box-binding protein 1 (YBX1) during adipogenesis of brown pre-adipocyte

SUBMITTER: Rabiee A 

PROVIDER: S-EPMC7779825 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature