Rapamycin and abundant TCR stimulation are required for the generation of stable human induced regulatory T cells.

ABSTRACT:

Objectives

Regulatory T cells (Tregs) are a vital sub-population of CD4⁺ T cells with major roles in immune tolerance and homeostasis. Given such properties, the use of regulatory T cells for immunotherapies has been extensively investigated, with a focus on adoptive transfer of ex vivo expanded natural Tregs (nTregs). For immunotherapies, induced Tregs (iTregs), generated in vitro from naïve CD4⁺ T cells, provide an attractive alternative, given the ease of generating cell numbers required for clinical dosage. While the combination of TGF-β, ATRA and rapamycin has been shown to generate highly suppressive iTregs, the challenge for therapeutic iTreg generation has been their instability. Here, we investigate the impact of rapamycin concentrations

SUBMITTER: Kim J 

PROVIDER: S-EPMC7780108 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature