Analysis of human satellite cell dynamics on cultured adult skeletal muscle myofibers.

ABSTRACT:

Background

Maintaining stem cells in physiologically relevant states is necessary to understand cell and context-specific signalling paradigms and to understand complex interfaces between cells in situ. Understanding human stem cell function is largely based on tissue biopsies, cell culture, and transplantation into model organisms.

Methods

Here, we describe a method to isolate post-mortem intact human muscle myofibers and culture muscle stem cells within the niche microenvironment to assay cellular dynamics, stem cell identity, stem cell hierarchy, and differentiation potential.

Results

We show human myofiber culture maintains complex cell-cell contacts and extracellular niche composition during culture. Human satellite cells can be cultured at least 8 days, which re

SUBMITTER: Feige P 

PROVIDER: S-EPMC7780694 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature