Simvastatin profoundly impairs energy metabolism in primary human muscle cells.

ABSTRACT:

Objectives

Simvastatin use is associated with muscular side effects, and increased risk for type 2 diabetes (T2D). In clinical use, simvastatin is administered in inactive lipophilic lactone-form, which is then converted to active acid-form in the body. Here, we have investigated if lactone- and acid-form simvastatin differentially affect glucose metabolism and mitochondrial respiration in primary human skeletal muscle cells.

Methods

Muscle cells were exposed separately to lactone- and acid-form simvastatin for 48 h. After pre-exposure, glucose uptake and glycogen synthesis were measured using radioactive tracers; insulin signalling was detected with Western blotting; and glycolysis, mitochondrial oxygen consumption and ATP production were measured with Seahorse XFe96 analyz

SUBMITTER: Makinen S 

PROVIDER: S-EPMC7780958 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature